abstract：:The number of the nucleoli in a CaCo-2 cell nucleus does not generally depend on the quantity of DNA in the nucleus, but nucleolar DNA content is directly proportional to total nuclear DNA. However, in multinucleolar cells (three or more nucleoli), the nucleolar DNA content increases after 96 h incubation in culture w...

journal_title：Cell biology international

authors： Karalyan ZA,Djaghatspanyan NG,Gasparyan MH,Hakobyan LA,Abroyan LO,Magakyan YH,Ter-Pogossyan ZR,Kamalyan LA,Karalova EM

更新日期：2004-01-01 00:00:00

abstract：:GDNF plays an important role in the survival and differentiation of primary dopaminergic neurons, but it requires multiple factors for its entire range of activities. This study investigated the effects of GDNF and its cofactors on the development of bFGF-responsive neural progenitor cells (NPCs), mesencephalic and co...

journal_title：Cell biology international

authors： Sun ZH,Lai YL,Li P,Zuo HC,Xie ZP

更新日期：2004-01-01 00:00:00

abstract：:Having the ability to control cell behaviour would be of great advantage in tissue engineering. One method of gaining control over cell adhesion, proliferation, guidance and differentiation is use of topography. Whilst it has be known for some time that cells can be guided by micro-topography, it is only recently beco...

journal_title：Cell biology international

authors： Dalby MJ,Riehle MO,Johnstone H,Affrossman S,Curtis AS

更新日期：2004-01-01 00:00:00

abstract：:Primary cilium development along with other components of the centrosome in mammalian cells was analysed ultrastructurally and by immunofluorescent staining with anti-acetylated tubulin antibodies. We categorized two types of primary cilia, nascent cilia that are about 1microm long located inside the cytoplasm, and tr...

journal_title：Cell biology international

authors： Alieva IB,Vorobjev IA

更新日期：2004-01-01 00:00:00

abstract：:Neural stem cells (NSCs) are currently considered powerful candidates for cell therapy in neurodegenerative disorders such as Parkinson's disease. However, it is not known when and how NSCs begin to differentiate functionally. Recent reports suggest that classical neurotransmitters such as acetylcholine (Ach) are invo...

journal_title：Cell biology international

authors： Zhou C,Wen ZX,Shi DM,Xie ZP

更新日期：2004-01-01 00:00:00

abstract：:Mitotic spindles of the diatom Pinnularia viritiformis stained with the fluorescent actin-labelling reagent bodipyphallacidin revealed actin among the chromosomes and extending along the spindle to the poles. This is the first report of actin's presence within spindles of this ecologically important group of organisms...

journal_title：Cell biology international

authors： Sampson K

更新日期：2004-01-01 00:00:00

abstract：:The objective of this study was to test the hypothesis that cytoskeletal actin fragmentation is mediated through caspase-2, specifically examining the ability of a caspase-2 inhibitor to interfere with actin fragmentation, in comparison with a caspase-3 inhibitor. Cardiomyocytes were cultured from embryonic chick hear...

journal_title：Cell biology international

authors： Kong JY,Rabkin SW

更新日期：2004-01-01 00:00:00

abstract：:Many disturbances in electrolyte and hormonal balance in the body induced by functional impairment of renal parenchyma may affect the activity of amine precursor uptake and decarboxylation (APUD) cells, which constitute a very important link in the regulation of homeostasis. The aim of the present study was the morpho...

journal_title：Cell biology international

authors： Kasacka I

更新日期：2004-01-01 00:00:00

abstract：:Metallothionein (MT), a cysteine-rich, metal-binding protein, is involved in homeostatic regulation of essential metals and protection of cells against oxidative injury. It has been shown that oxidative stress is associated with pathogenesis of osteoporosis and is capable of inhibiting osteoblastic differentiation of ...

journal_title：Cell biology international

authors： Liu AL,Zhang ZM,Zhu BF,Liao ZH,Liu Z

更新日期：2004-01-01 00:00:00

abstract：:Epidermis, a continuously renewing tissue, is maintained by stem cells that proliferate and replenish worn out or damaged cells in the tissue during life. Cultured epidermal stem cells have great potential in scientific research and clinical application. However, isolating a pure and viable population of epidermal ste...

journal_title：Cell biology international

authors： Zhou JX,Jia LW,Yang YJ,Peng S,Cao YJ,Duan EK

更新日期：2004-01-01 00:00:00

abstract：:Using flow cytometry and confocal microscopy, the presence of endorphin, serotonin and chorionic gonadotropin (hCG) was demonstrated in rat white blood cells and peritoneal mast cells. After a single neonatal treatment with beta-endorphin (hormonal imprinting), the mast cells of female rats reaching adulthood containe...

journal_title：Cell biology international

authors： Csaba G,Kovács P,Pállinger E

更新日期：2003-01-01 00:00:00

abstract：:The effects of melanin preparations from black yeast fungi (BYF) on the proliferation and differentiation of normal cultivated human skin keratinocytes and embryonic pulmonary fibroblasts have been investigated. Melanin preparations in the range of 5-0.1 microg/ml were optimally active, with a more pronounced effect o...

journal_title：Cell biology international

authors： Blinova MI,Yudintseva NM,Kalmykova NV,Kuzminykh EV,Yurlova NA,Ovchinnikova OA,Potokin IL

更新日期：2003-01-01 00:00:00

abstract：:Mutants of the methanol-utilizing yeast Pichia pastoris and the alkane-utilizing yeast Yarrowia lipolytica defective in the orthologue of UGT51 (encoding sterol glucosyltransferase) were isolated and compared. These mutants do not contain the specific ergosterol derivate, ergosterol glucoside. We observed that the P. ...

journal_title：Cell biology international

authors： Stasyk OV,Nazarko TY,Stasyk OG,Krasovska OS,Warnecke D,Nicaud JM,Cregg JM,Sibirny AA

更新日期：2003-01-01 00:00:00

abstract：:The aim of this study was to examine the effects of glafenine hydrochloride (a nonsteroidal anti-inflammatory drug) on proliferation, clonogenic activity, cell-cycle, migration, and the extracellular matrix protein tenascin of human aortic smooth muscle cells (haSMCs) and human endothelial cells (ECs) in vitro.HaSMCs ...

journal_title：Cell biology international

authors： Schöber W,Tran QB,Muringaseril M,Wiskirchen J,Kehlbach R,Rodegerdts E,Wiesinger B,Claussen CD,Duda SH

更新日期：2003-01-01 00:00:00

abstract：:Properly metabolized globin synthesis and iron uptake are indispensable for erythroid cell differentiation and maturation. Mitochondrial participation is crucial in the process of haeme synthesis for cytochromes and haemoglobin. We studied the final biosynthesis site of haemoglobin using an ultrastructural approach, w...

journal_title：Cell biology international

authors： Cianciarullo AM,Bertho AL,Soares MJ,Hosoda TM,Nogueira-Silva S,Beçak W

更新日期：2003-01-01 00:00:00

abstract：:We analyzed the connection of changes in nucleus ploidy with changes in nucleolar apparatus of NIH 3T3 cells. The quantity of nucleoli does not depend on the quantity of nucleolar DNA, but instead depends on euploidy: the majority of euploid cells have 1-3 nucleoli. The quantity of DNA in the nucleolus is correlated w...

journal_title：Cell biology international

authors： Karalyan ZA,Djaghatspanyan NG,Gasparyan MH,Hakobyan LA,Abroyan LO,Magakyan YH,Ter-Pogossyan ZR,Kamalyan LA,Avagyan VY,Karalova EM

更新日期：2003-01-01 00:00:00

abstract：:Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a key enzyme of the glycolytic pathway. Recent studies have demonstrated an additional role in apoptosis: GAPDH is targeted to the nucleus during apoptotic signalling. This nuclear transport has also been observed in serum-depleted cells, but it is reversible in fibr...

journal_title：Cell biology international

authors： Schmitz HD,Dutiné C,Bereiter-Hahn J

更新日期：2003-01-01 00:00:00

abstract：:Extracellular nucleotides cause neutrophil degranulation by activating the purinergic receptor subtype P2Y. However, the molecular mechanism involved in the signal pathway remains unknown. A hypothetical scheme suggesting that leukotriene(s) and leukotriene receptor(s) activation is required for extracellular nucleoti...

journal_title：Cell biology international

authors： Kannan S

更新日期：2003-01-01 00:00:00

abstract：:Bradykinin (1 microM) and histamine (100 microM) evoked an initial transient increase and a subsequent sustained increase in intracellular Ca(2+) concentration ([Ca(2+)](i)) in fura-2-loaded human gingival fibroblasts, which may be attributed to Ca(2+) release from intracellular stores and Ca(2+) entry from extracellu...

journal_title：Cell biology international

authors： Ogata Y,Nakao S,Shimizu E,Matsuda-Honjyo Y,Yamazaki M,Furuyama S,Sugiya H

更新日期：2003-01-01 00:00:00

abstract：:Human myometrial smooth muscle cells (SMCs) were used to evaluate the proliferative activity of lysophosphatidic acid (LPA). This study specifically focuses on the role of Ca(2+)/calmodulin-dependent protein (CaM) kinase and epidermal growth factor (EGF) receptor tyrosine kinase. Myometrial SMCs were cultured from bio...

journal_title：Cell biology international

authors： Nilsson UK,Svensson SP

更新日期：2003-01-01 00:00:00

abstract：:Under the 1G condition, the increase in antipain-sensitive protease activity promptly after UV (mainly 254 nm wavelength) irradiation in cultured human cells is detected and found to be one of the intriguing events involved in suppression of cell mutability. It was found that two cell lines, RSa and its variant UVAP-1...

journal_title：Cell biology international

authors： Takahashi S,Zhang HC,Hasegawa R,Karata K,Kita K,Wano C,Yamaguchi Y,Sugaya S,Nomura J,Ichinose M,Suzuki N

更新日期：2003-01-01 00:00:00

abstract：:This review outlines the basic properties of T-tubules in skeletal muscle cells, and the factors that govern reversible vacuolation in T-tubules under experimental conditions. Comparable membranous transformations, involving the plasma membrane or occurring intracellularly, in non-muscle cells are then considered. Fin...

journal_title：Cell biology international

authors： Krolenko SA,Lucy JA

更新日期：2002-01-01 00:00:00

abstract：:We have previously reported that the T cell line Jurkat registers the exposure of a sinusoidal extremely low frequency magnetic field at the level of the plasma membrane, resulting in activation of the tyrosine kinase p56(lck), increase in inositol-3-phosphate levels and increase in intracellular calcium concentration...

journal_title：Cell biology international

authors： Still M,Lindström E,Ekstrand AJ,Mild KH,Mattsson MO,Lundgren E

更新日期：2002-01-01 00:00:00

abstract：:Increasing potassium chloride concentration from 0 to 100 mM and magnesium chloride from 0 to 2 mM show a parallel rate increase in polymerizing actin, whereas increasing calcium chloride concentration from 0 to 0.2 mM decreases the rate of polymerizing actin. The presence of alpha-actinin has little influence on the ...

journal_title：Cell biology international

authors： Goldmann WH

更新日期：2002-01-01 00:00:00

abstract：:Cell-matrix and cell-cell interactions are important physiological determinants of cell growth, survival and transformation. Cell adhesion to the extra cellular matrix (ECM) via integrins also crucially influences the organization of the cytoskeleton. It triggers a cascade of intracellular biochemical events, which re...

journal_title：Cell biology international

authors： Rajeswari J,Pande G

更新日期：2002-01-01 00:00:00

abstract：:Cadherins, being responsible for selective cell recognition and normal tissue integrity in adults, regulate morphogenesis in a variety of organs during development. In this study, anti-rat pan-cadherin antibody, specific to all subgroups of the cadherin family, was used to map the distribution of the pan-cadherin immu...

journal_title：Cell biology international

authors： Filiz S,Dalcik H,Yardimoglu M,Gonca S,Ceylan S

更新日期：2002-01-01 00:00:00

abstract：:A subcellular fractionation procedure was developed to isolate the different endosomal compartments present during reticulocyte maturation. After reticulocyte lysis and removal of excess haemoglobin by gel chromatography, membrane vesicles were separated over a discontinuous sucrose gradient (10-40%). Two fractions we...

journal_title：Cell biology international

authors： Dardalhon V,Géminard C,Reggio H,Vidal M,Sainte-Marie J

更新日期：2002-01-01 00:00:00

abstract：:The effects of cadmium Cd (II) ions on the physiology and biological activity of Trametes versicolor, a strain belonging to white-rotting Basidiomycetes, were examined. Cd (II) ions were added to 10-day-old cultures grown on a liquid medium, or at the time of inoculation. Our experiments showed that T. versicolor is a...

journal_title：Cell biology international

authors： Jarosz-Wilkolazka A,Malarczyk E,Pirszel J,Skowroński T,Leonowicz A

更新日期：2002-01-01 00:00:00

abstract：:Tropical pulmonary eosinophilia is in part caused by the hyperimmune responsiveness of the lung tissue against the antigens of degenerating microfilariae. We have previously shown that the activation of the transcription factor NF-kappaB is essential for the synthesis and release of multiple pro-inflammatory cytokines...

journal_title：Cell biology international

authors： Narayanan K,Seufzer BJ,Brockman-Schneider RA,Gern JE,Balakrishnan A,Miyamoto S

更新日期：2002-01-01 00:00:00

abstract：:The dynamic state of post-translational acetylation of eukaryotic histones is maintained by histone acetyltransferases (HATs) and histone deacetylases (HDACs). HATs and HDACs have been shown to be components of various regulatory protein complexes in the cell. Their enzymatic activities, intracellular localization and...

journal_title：Cell biology international

authors： Brandtner EM,Lechner T,Loidl P,Lusser A

更新日期：2002-01-01 00:00:00

abstract：:Continuous variation in the direction of the gravity vector leads to various cellular responses including modulation of gene expression. Complementary DNA (cDNA) array analyses are available to observe the variation of gene expression under different conditions. In this study, expression levels of 588 representative g...

journal_title：Cell biology international

authors： Arase Y,Nomura J,Sugaya S,Sugita K,Kita K,Suzuki N

更新日期：2002-01-01 00:00:00

abstract：:Physical forces play a critical role in cell integrity and development, but little is known how cells convert mechanical signals into biochemical responses. This mini-review examines potential molecular mediators like integrins, focal adhesion proteins, and the cytoskeleton in the context of a complex cell structure. ...

journal_title：Cell biology international

authors： Goldmann WH

更新日期：2002-01-01 00:00:00

abstract：:Prolapsus uteri in pelvic supportive disorders are common in elderly women, and their etiology remains unclear. We examined elastin-binding proteins (EBPs) and binding sites in cultured cardinal ligament fibroblasts derived from elderly patients with prolapsus uteri (HPLiF) and compared them with those from age-matche...

journal_title：Cell biology international

authors： Yamamoto M,Akazawa K,Aoyagi M,Yamamoto N,Yamamoto K

更新日期：2002-01-01 00:00:00

abstract：:Bcl-2 family proteins play a critical role in the regulation of apoptosis. Treatment of a human hepatocellular carcinoma cell line, QGY-7703, with Taxol induced apoptosis and Bcl-2 protein phosphorylation. Microscopic observation indicated that apoptotic bodies (0-15%) of Taxol-treated QGY cells appeared after 12 h of...

journal_title：Cell biology international

authors： Cheng SC,Luo D,Xie Y

更新日期：2001-01-01 00:00:00

abstract：:Non-transformed epitheliocytes and fibroblasts undergo modulations between the less polarised and more polarised phenotypes depending on the nature of the substrate. In contrast, transformed cells express polarised phenotype regardless of the substrate. ...

journal_title：Cell biology international

authors： Moizhess TG,Vasiliev JM

更新日期：2001-01-01 00:00:00

abstract：:The advent of scanning probe microscopy has introduced a powerful new method of probing the structural features of biological specimens. In this study, high resolution atomic force microscopy micrographs of single, isolated, cardiac myocytes are presented. Significantly, our images show not only the features to be exp...

journal_title：Cell biology international

authors： Davis JJ,Hill HA,Powell T

更新日期：2001-01-01 00:00:00

abstract：:Normal human bone marrow stroma cells include stem cells for both haemopoietic and osteochondrogenic lineages and express both bone morphogenetic protein (BMP) type I and type II receptors. As a member of the TGF-beta super-family, BMP-6 binds to both BMP type I and type II receptors and is involved in the development...

journal_title：Cell biology international

authors： Ahmed N,Sammons J,Carson RJ,Khokher MA,Hassan HT

更新日期：2001-01-01 00:00:00

abstract：:Annexin I is an abundant cytosolic protein in human neutrophils. Besides its intracellular location, annexin I is found as an extracellular protein and the pathway for secretion has been of interest since the protein lacks a signal sequence for secretion. It was recently shown that annexin I is stored in the secretory...

journal_title：Cell biology international

authors： Movitz C,Dahlgren C

更新日期：2001-01-01 00:00:00

abstract：:We have studied the effect of enhanced expression of epithelial Na(+)channels (ENaC) on the ATP-induced Cl(-)secretion in the mouse epithelium using short-circuit current (I(SC)) and RT-PCR techniques. The amiloride sensitivity of basal current (I(b)) across the cultured endometrial epithelia was found to vary with th...

journal_title：Cell biology international

authors： Wang XF,Tsang LL,So SC,Chan HC

更新日期：2001-01-01 00:00:00

abstract：:Epicatechin, a flavonoid belonging to the group of compounds collectively called catechins, have been reported to possess insulin-like properties. Besides their anti-diabetic properties, catechins also show growth inhibition. Since cytosolic pH (pHi) plays a role in cell proliferation and the Na/H exchanger (NHE) is t...

journal_title：Cell biology international

authors： Matteucci E,Rizvi SI,Giampietro O

更新日期：2001-01-01 00:00:00